Gene description for CD63
Gene name CD63 molecule
Gene symbol CD63
Other names/aliases AD1
HOP-26
ME491
MLA1
OMA81H
Pltgp40
TSPAN30
Species Homo sapiens
 Experiment description of studies that identified CD63 in Dendritic cells

1
Experiment ID50
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method FACS
Immunoelectron Microscopy
PubMed ID 14582906   
OrganismHomo sapiens
Experiment descriptionExosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
AuthorsAdmyre C, Grunewald J, Thyberg J, Gripenbä S, Tornling G, Eklund A, Scheynius A, Gabrielsson S
Journal name ERJ
Publication year2003
SampleDendritic cells
Sample nameMonocyte-derived dendritic cells
Isolation/purification methodsDifferential centrifugation
Immunobeads (EpCAM)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
Immunoelectron microscopy
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2
Experiment ID117
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 21505438   
OrganismHomo sapiens
Experiment descriptionUnidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
AuthorsMittelbrunn M, Gutiéez-Váuez C, Villarroya-Beltri C, Gonzáz S, Sáhez-Cabo F, Gonzáz MÁ Bernad A, Sáhez-Madrid F.
Journal name NATCOM
Publication year2011
SampleDendritic cells
Sample nameDendritic cells
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
Microarray
EV-TRACK EV110049: EV-METRIC:11%

3
Experiment ID270
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Immunoelectron microscopy
PubMed ID 10903724   
OrganismHomo sapiens
Experiment descriptionFollicular dendritic cells carry MHC class II-expressing microvesicles at their surface
AuthorsDenzer K, van Eijk M, Kleijmeer MJ, Jakobson E, de Groot C, Geuze HJ
Journal name J Immunol
Publication year2000
SampleDendritic cells
Sample nameFollicular dendritic cells
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyImmunoelectron microscopy
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4
Experiment ID562
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F3 10K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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5
Experiment ID563
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F5 10K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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6
Experiment ID564
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F3 100K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -

7
Experiment ID565
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry [LTQ ORBITRAP]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F5 100K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ ORBITRAP]
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8
Experiment ID570
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ FUSION]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (CD63 PD)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Immunobeads (CD63 PD)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ FUSION]
EV-TRACK -

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